Directed PCR-free engineering of highly repetitive DNA sequences
نویسندگان
چکیده
منابع مشابه
Directed PCR-free engineering of highly repetitive DNA sequences
BACKGROUND Highly repetitive nucleotide sequences are commonly found in nature e.g. in telomeres, microsatellite DNA, polyadenine (poly(A)) tails of eukaryotic messenger RNA as well as in several inherited human disorders linked to trinucleotide repeat expansions in the genome. Therefore, studying repetitive sequences is of biological, biotechnological and medical relevance. However, cloning of...
متن کاملDNA Fingerprinting Based on Repetitive Sequences of Iranian Indigenous Lactobacilli Species by (GTG)5- REP-PCR
Background and Objective: The use of lactobacilli as probiotics requires the application of accurate and reliable methods for the detection and identification of bacteria at the strain level. Repetitive sequence-based polymerase chain reaction (rep-PCR), a DNA fingerprinting technique, has been successfully used as a powerful molecular typing method to determine taxonomic and phylogenetic relat...
متن کاملHighly repetitive DNA sequences in cyanobacterial genomes.
We characterized three distinct families of repeated sequences in the genome of the cyanobacterium Calothrix sp. strain PCC 7601. These repeated sequences were present at a level of about 100 copies per Calothrix genome and consisted of tandemly amplified heptanucleotides. These elements were named short tandemly repeated repetitive (STRR) sequences. We used the three different Calothrix STRR s...
متن کاملHighly Repetitive Satellite DNA
DNA Sequence, Satellite DNA, Cucumis melo The primary sequence of the prominent Cucumis melo satellite DNA is presented. The cloned Hind Ill-repeat unit has a length of 352 bp and a GC-content of 56%. Within this sequence an inverted repeat of 9 bp, including a 286 bp loop, and one direct repeat are shown. Several open reading frames are possible following the sequence in both directions whose ...
متن کاملJunk DNA - repetitive sequences
Eukaryote and also human DNA contains large portion of noncoding sequences. As for the coding DNA, the noncoding DNA may be unique or in more identical or similar copies. DNA sequences with high copy numbers are then called repetitive sequences. If the copies of a sequence motif lie adjacent to each other in a block, or an array, we are speaking about tandem repeats, the repetitive sequences di...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Biotechnology
سال: 2011
ISSN: 1472-6750
DOI: 10.1186/1472-6750-11-87